IL-11 Antibody

ProSci
Product Code: PSI-XP-5164Bt
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5164Bt-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
IL-11 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect hIL-11 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human IL-11 (XP-5164) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hIL-11.

To detect hIL-11 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human IL-11 (XP-5164) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hIL-11.

Further Information

Additional Names:
AGIF, IL-11Interleukin-11, Adipogenesis inhibitory factor, IL-11
Application Note:
ELISA:

Sandwich:
To detect hIL-11 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-11 (XP-5164) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-11.

Western Blot:

To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Interleukin 11 is a pleiotropic cytokine produced by mesenchymal-derived adherent cells. IL11 shares many functions of IL6 and LIF, including potentiation of megakaryocyte activity, enhancement of human myeloma cell proliferation, and enhancement of hepatic acute phase protein production. Interleukin 11 directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production. Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelosuppressive chemotherapy.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-11 (human IL-11).
NCBI Gene ID #:
3589
NCBI Official Name:
interleukin 11
NCBI Official Symbol:
IL11
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P20809
Protein GI Number:
124294
Purification:
Anti-hIL-11 specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Immunology,Chemokines & Cytokines,Neuroscience,Antibody Pairs
Swissprot #:
P20809
User NOte:
Centrifuge vial prior to opening.