MIP-3B Antibody

ProSci
Product Code: PSI-XP-5241
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5241-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
MIP-3B antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze

Images

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To detect Human MIP-3-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MIP-3-beta (XP-5241Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3-beta.
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To detect Human MIP-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect Human MIP-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human MIP-3-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human MIP-3-beta (XP-5241Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIP-3-beta.
To detect Human MIP-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human MIP-3-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3-beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Further Information

Additional Names:
CKb4, LARC, ST38, MIP3A, MIP-3a, SCYA20, MIP-3-alpha, C-C motif chemokine 20, Beta-chemokine exodus-1
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-3β (100 ng/mL), a concentration of 2.7 4.0 μg/mL of this antibody is required.

ELISA:

To detect hMIP-3β by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-3β.

Sandwich:

To detect hMIP-3β by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-3β (XP-5241Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-3β.

Western Blot:
To detect hMIP-3β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-3β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-3β (human Macrophage Inflammatory Protein-3 beta).
NCBI Gene ID #:
6364
NCBI Official Name:
chemokine (C-C motif) ligand 20
NCBI Official Symbol:
CCL20
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P78556
Protein GI Number:
2829668
Purification:
Anti-hMIP-3β specific antibody was purified by affinity chromatography employing immobilized hMIP-3β matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P78556
User NOte:
Centrifuge vial prior to opening.