OncostatinM Antibody
Code | Size | Price |
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PSI-XP-5252-0.1mg | 0.1mg | £524.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
OncostatinM antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated f
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Further Information
Additional Names:
Oncostatin-MOSM
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hOncostatin-M (10 ng/mL), a concentration of 0.100 - 0.175 μg/mL of this antibody is required.
ELISA:
To detect hOncostatin-M by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hOncostatin-M.
Sandwich:
To detect hOncostatin M by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Oncostatin M (XP-5252Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOncostatin M.
Western Blot:
To detect hOncostatin-M by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin-M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hOncostatin-M (10 ng/mL), a concentration of 0.100 - 0.175 μg/mL of this antibody is required.
ELISA:
To detect hOncostatin-M by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hOncostatin-M.
Sandwich:
To detect hOncostatin M by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human Oncostatin M (XP-5252Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOncostatin M.
Western Blot:
To detect hOncostatin-M by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin-M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hOncostatin-M (human Oncostatin-M).
NCBI Gene ID #:
5008
NCBI Official Name:
oncostatin M
NCBI Official Symbol:
OSM
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P13725
Protein GI Number:
129168
Purification:
Anti-hOncostatin-M specific antibody was purified by affinity chromatography employing immobilized hOncostatin-M matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P13725
User NOte:
Centrifuge vial prior to opening.