Perforin (CB5.4) Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-XA-1004-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rat
Antibody Isotype: Rat IgG2a
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Immunocytochemistry (ICC)
- Immunohistochemistry (IHC)
- Immunoprecipitation (IP)
- Western Blot (WB)
Storage:
Perforin antibody can be stored at 4˚C, stable for one year.
Documents
Further Information
Application Note:
Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL. This product can be used for detection of active human FasL by flow cytometry and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 μg/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system.
Background References:
- The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993).
- Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller and J. Tschopp; J. Immunol. 153, 2470 (1994).
- Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002).
- Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.
NCBI Gene ID #:
18646
NCBI Official Name:
perforin 1 (pore forming protein)
NCBI Official Symbol:
Prf1
NCBI Organism:
Mus musculus
Physical State:
Liquid
Purification:
Perforin (monoclonal CB5.4) antibody was purified to ≥95% in SDS-PAGE.
Research Area:
Immunology
Swissprot #:
P10820
User NOte:
Optimal dilutions for each application to be determined by the researcher.