TWEAK Antibody

ProSci
Product Code: PSI-XP-5290Bt
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5290Bt-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
TWEAK antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect hTWEAK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human TWEAK (XP-5290) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hTWEAK.

To detect hTWEAK by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Human TWEAK (XP-5290) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant hTWEAK.

Further Information

Additional Names:
APO3L, DR3LG, TWEAK, APO3L, UNQ181/PRO207, Tumor necrosis factor ligand superfamily member 12, APO3 ligand
Application Note:
ELISA:

Sandwich:
To detect hTWEAK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human TWEAK (XP-5290) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTWEAK.

Western Blot:

To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTWEAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
TWEAK is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hTWEAK (human TWEAK).
NCBI Gene ID #:
8742
NCBI Official Name:
tumor necrosis factor (ligand) superfamily, member 12
NCBI Official Symbol:
TNFSF12
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O43508
Protein GI Number:
21362987
Purification:
Anti-hTWEAK specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Cancer,Apoptosis,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
O43508
User NOte:
Centrifuge vial prior to opening.