HSPA8 Antibody

ProSci
Product Code: PSI-45-072
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-072-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-072 (0.03ug/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-072 (0.03ug/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
HSPA8 , heat shock 70kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat shock 70kd protein 10, heat shock cognate protein 54, heat shock cognate protein, 71-kDa, lipopolysaccharide-associated protein , uncharacterized bone marrow protein BM034
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 75kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9kDa according to Human NP_006588.1 and Mouse NP_112442.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence:?Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml
Background References:
  • Zhang Z, Fan J, Becker KG, Graff RD, Lee GM, Francomano CA. Comparison of gene expression profile between human chondrons and chondrocytes: a cDNA microarray study. Osteoarthritis Cartilage. 2006 May;14(5):449-59. Epub 2006 Jan 18.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-EKLQGKINDEDKQK
NCBI Gene ID #:
3312
NCBI Official Name:
heat shock 70kDa protein 8
NCBI Official Symbol:
HSPA8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006588.1
Protein GI Number:
5729877
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_006588.1) only.
Swissprot #:
P11142