FGF-10 Antibody

ProSci
Product Code: PSI-XP-5131
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5131-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
FGF-10 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze

Images

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This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml - 2.5 ug/ml with an overnight iincubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
2 / 3
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml - 2.5 ug/ml with an overnight iincubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
3 / 3
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml - 2.5 ug/ml with an overnight iincubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml - 2.5 ug/ml with an overnight iincubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml - 2.5 ug/ml with an overnight iincubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 1.0 ug/ml - 2.5 ug/ml with an overnight iincubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
Fibroblast growth factor 10, Keratinocyte growth factor 2, FGF-10
Application Note:
ELISA:

To detect hFGF-10 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-10.

Sandwich: To detect hFGF-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human FGF-10 (XP-5131Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-10.

Western Blot:

To detect hFGF-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFGF-10 (human Fibroblast Growth Factor-10).
NCBI Gene ID #:
2255
NCBI Official Name:
fibroblast growth factor 10
NCBI Official Symbol:
FGF10
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
O15520
Protein GI Number:
6015141
Purification:
Anti-hFGF-10 specific antibody was purified by affinity chromatography employing immobilized hFGF-10 matrix.
Research Area:
Growth Factor,Antibody Pairs
Swissprot #:
O15520
User NOte:
Centrifuge vial prior to opening.