MCP-1 Antibody

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ProSci
Product Code: PSI-XP-5732-M
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5732-M-0.5mg0.5mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
MCP-1 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4&

Images

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To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 300 pg/ml of recombinant human MCP-1 when used with ProSci's biotinylated antigen affinity purified anti-human MCP-1 (XP-5218Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.
2 / 6
To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
3 / 6
To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
4 / 6
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
5 / 6
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
6 / 6
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
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To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 300 pg/ml of recombinant human MCP-1 when used with ProSci's biotinylated antigen affinity purified anti-human MCP-1 (XP-5218Bt) as the detection antibody at a concentration of approximately 0.5-1.0 ug/ml.
To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.

Documents

Further Information

Additional Names:
MCP1, SCYA2, C-C motif chemokine 2, HC11, MCAF, MCP-1, GDCF-2, SMC-CF, HSMCR30
Application Note:
ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 300 pg/mL of recombinant human MCP-1 when used with our biotinylated antigen affinity purified anti-human MCP-1 (XP-5218Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Western Blot:
To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hMCP-1/MCAF as the immunizing antigen.
NCBI Gene ID #:
6347
NCBI Official Name:
chemokine (C-C motif) ligand 2
NCBI Official Symbol:
CCL2
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P13500
Protein GI Number:
126842
Purification:
This IgG1/K antibody was purified from ascites fluid on a Protein A affinity column.
Research Area:
Chemokines & Cytokines
Swissprot #:
P13500
User NOte:
Centrifuge vial prior to opening.