CD31 Monoclonal Antibody

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ProSci
Product Code: PSI-32-131
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-131-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 2F7B2
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
CD31 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using CD31 monoclonal antibody against truncated CD31 recombinant protein.
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Western blot analysis using CD31 monoclonal antibody against truncated CD31 recombinant protein.

Documents

Further Information

Additional Names:
EndoCAM, PECAM-1, CD31, PECA1, GPIIA', endoCAM, CD31/EndoCAM
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells and platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
Background References:
  • Mayr U et al. Circ Res 98:412-20 (2006).
  • Bingle L et al. Br J Cancer 94:101-7 (2006).
  • Wynne F et al. Reproduction 131:721-32 (2006).
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant CD31 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
5175
NCBI Official Name:
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol:
PECAM1
NCBI Organism:
Homo sapiens
Protein Accession #:
P16284
Protein GI Number:
129747
Research Area:
Innate Immunity
Swissprot #:
P16284
User NOte:
Optimal dilutions for each application to be determined by the researcher.