CD4 Monoclonal Antibody

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ProSci
Product Code: PSI-32-133
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-133-0.1mg0.1mg£416.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: B486A1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
CD4 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD4.
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Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD4.

Documents

Further Information

Additional Names:
CD4mut
Application Note:
Western Blot, IHC and ELISA.
Dilution:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
Background:
The CD4 antigen is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. This integral membrane glycoprotein of approximately 58 kDa contains four external domains (D1 to D4) that show homology to members of the immunoglobulin (Ig) superfamily. It is responsible for the recognition of the MHC class II antigen. The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells.
Background References:
  • M Benkirane J Virol. 1995 November; 69(11): 6898?C6903.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human CD4 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
920
NCBI Official Name:
CD4 molecule
NCBI Official Symbol:
CD4
NCBI Organism:
Homo sapiens
Protein Accession #:
NP_000607.1
Protein GI Number:
10835167
Purification:
Antibody is purified by protein A affinity chromatography.
Research Area:
Immunology ,
Swissprot #:
P01730
User NOte:
Optimal dilutions for each application to be determined by the researcher.