KGF Antibody
| Code | Size | Price |
|---|
| PSI-XP-5208-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
KGF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-th
Documents
Further Information
Additional Names:
KGF, HBGF-7, KGF, Fibroblast growth factor 7, Heparin-binding growth factor 7, FGF-7
Application Note:
ELISA:
To detect hKGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hKGF.
Sandwich:
To detect hKGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human KGF (XP-5208Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hKGF.
Western Blot:
To detect hKGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hKGF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hKGF.
Sandwich:
To detect hKGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human KGF (XP-5208Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hKGF.
Western Blot:
To detect hKGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hKGF (human Keratinocyte Growth Factor).
NCBI Gene ID #:
2252
NCBI Official Name:
fibroblast growth factor 7
NCBI Official Symbol:
FGF7
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P21781
Protein GI Number:
122756
Purification:
Anti-hKGF specific antibody was purified by affinity chromatography employing immobilized hKGF matrix.
Research Area:
Growth Factor,Antibody Pairs
Swissprot #:
P21781
User NOte:
Centrifuge vial prior to opening.