MCP-4 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5731-M-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
MCP-4 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4&
Documents
Further Information
Additional Names:
macrophage, monocyte chemotactic protein 4, MCP4, NCC1, SCYA13, C-C motif chemokine 13, CK-beta-10, MCP-4, CKb10, NCC-1, SCYL1
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rMCP-4 (100 ng/mL), a concentration of 1-5 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-4 when used with our biotinylated antigen affinity purified anti-human MCP-4 (XP-5225Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of rMCP-4 (100 ng/mL), a concentration of 1-5 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-4 when used with our biotinylated antigen affinity purified anti-human MCP-4 (XP-5225Bt) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2.0-4.0 ng/lane, under non-reducing conditions.
Background:
MCP4 is a member of the b-chemokine family (C-C) of Cytokines. Like Eotaxin, MCP4 is a powerful eosinophil chemo-attractant. Additionally, like MCP-3, MCP-4 elicits chemo-attraction of monocytes and T lymphocytes
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c mice using recombinant human MCP-4 as the immunizing antigen.
NCBI Gene ID #:
6357
NCBI Official Name:
chemokine (C-C motif) ligand 13
NCBI Official Symbol:
CCL13
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q99616
Protein GI Number:
3024128
Purification:
This IgG1/K antibody was purified from a Protein A chromatography column.
Research Area:
Chemokines & Cytokines, Functional Assays
Swissprot #:
Q99616
User NOte:
Centrifuge vial prior to opening.