VEGF Antibody
| Code | Size | Price |
|---|
| PSI-XP-5740-M-0.5mg | 0.5mg | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
VEGF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4&#
Documents
Further Information
Additional Names:
Vascular Endothelial Growth Factor, VEGF, Vascular endothelial growth factor A, Vascular permeability factor, VEGF-A,
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of <0.2 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μg/mL.
Western Blot:
To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10.0 ng/mL), a concentration of <0.2 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100uL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with our biotinylated antigen affinity purified anti-human VEGF165 (XP-5291Bt) as the detection antibody at a concentration of approximately 0.25-1.0 μg/mL.
Western Blot:
To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human VEGF as the immunizing antigen.
NCBI Gene ID #:
7422
NCBI Official Name:
vascular endothelial growth factor A
NCBI Official Symbol:
VEGFA
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P15692
Protein GI Number:
17380528
Purification:
This IgG1k antibody was purified from ascites fluid followed by Protein A affinity chromatography.
Research Area:
Signal Transduction,Growth Factor, Functional Assays
Swissprot #:
P15692
User NOte:
Centrifuge vial prior to opening.