IFN-gamma Antibody

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ProSci
Product Code: PSI-XP-5739-M
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5739-M-0.5mg0.5mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
IFN-γ lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at

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To a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human IFN-gamma when used with ProSci's biotinylated antigen affinity purified anti-human IFN-gamma (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.
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To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.
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To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.
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To a sandwich ELISA (assuming 100ul/well), a concentration of 4.0-8.0 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human IFN-gamma when used with ProSci's biotinylated antigen affinity purified anti-human IFN-gamma (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 ug/ml.
To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.
To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.

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Further Information

Additional Names:
AF1, Interferon gamma receptor , Interferon gamma transducerInterferon gamma, Immune interferon, IFN-gamma, IFG, IFI
Application Note:
ELISA:

In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human IFN-γ when used with our biotinylated antigen affinity purified anti-human IFN-γ (XP-5155Bt) as the detection antibody at a concentration of approximately 2.0-4.0 μg/mL.

Western Blot:

To detect hIFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIFN-γ is 1.0-3.0 ng/lane, under non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
The monoclonal antibody was produced in BALB/c x ICR F1 mice using recombinant IFN-gamma as the immunizing antigen.
NCBI Gene ID #:
3458
NCBI Official Name:
interferon, gamma
NCBI Official Symbol:
IFNG
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P01579
Protein GI Number:
124479
Purification:
This IgG1k antibody was purified on a Protein A affinity column.
Research Area:
Immunology,Chemokines & Cytokines
Swissprot #:
P01579
User NOte:
Centrifuge vial prior to opening.