Gro-gamma Antibody

ProSci
Product Code: PSI-XP-5150
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5150-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
Gro-gamma antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fre

Images

1 / 5
To detect Human GRO-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human GRO-gamma (XP-5150Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO-gamma.
2 / 5
To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 5
To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 5
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/ml-0.5mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
5 / 5
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/ml-0.5mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

To detect Human GRO-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human GRO-gamma (XP-5150Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO-gamma.
To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/ml-0.5mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/ml-0.5mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
GRO3, GROg, MIP2B, SCYB3, MIP-2b, CINC-2b, GRO3, GROG, C-X-C motif chemokine 3, Growth-regulated protein gamma, GRO-gamma
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-gamma (100 ng/mL), a concentration of 1.0 - 2.0 μg/mL of this antibody is required.

ELISA:

To detect hGRO-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO-gamma.

Sandwich: To detect hGRO-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO-γ (XP-5150Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-γ.

Western Blot:
To detect hGRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO-gamma (human GRO-gamma).
NCBI Gene ID #:
2921
NCBI Official Name:
chemokine (C-X-C motif) ligand 3
NCBI Official Symbol:
CXCL3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P19876
Protein GI Number:
127086
Purification:
Anti-hGRO-gamma specific antibody was purified by affinity chromatography employing immobilized hGRO-gamma matrix.
Research Area:
Chemokines & Cytokines,Signal Transduction,Homeostasis,Antibody Pairs, Functional Assays
Swissprot #:
P19876
User NOte:
Centrifuge vial prior to opening.