Chromogranin A Antibody

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ProSci
Product Code: PSI-45-039
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-039-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-039 (0.1ug/ml) staining of isoform 1+ 2 Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-039 (2ug/ml) staining of isoform 1 Human Brain (A) and isoform 1+2 (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-039 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol
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45-039 (0.1ug/ml) staining of isoform 1+ 2 Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-039 (2ug/ml) staining of isoform 1 Human Brain (A) and isoform 1+2 (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-039 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol

Documents

Further Information

Additional Names:
chromogranin A precursor, chromogranin A (parathyroid secretory protein 1) , CHGA, CGA , parathyroid secretory protein 1, Vasostatin, Pancreastatin, Parastatin, Pituitary secretory protein I, SP1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 50kDa band observed in Human Cerebellum and Kidney lysates. An additional band at 35kDa was observed in some lysates (calculated MW of 50.7 kDa according to NP_001266.1 isoform 1 and 34.3kDa according to NP_001288619.1 isoform 2). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Pancreas and Small Intestine. Recommended concentration: 3.75ug/ml.Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.
Background References:
  • Li L, Yuan YZ, Lu J, Xia L, Zhu Y, Zhang YP, Qiao MM. Treatment of pancreatic carcinoma by adenoviral mediated gene transfer of vasostatin in mice. Gut. 2006 Feb;55(2):259-65. Epub 2005 Nov 15.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QGAKERAHQQKKH
NCBI Gene ID #:
1113
NCBI Official Name:
chromogranin A (parathyroid secretory protein 1)
NCBI Official Symbol:
CHGA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001266.1, NP_001288619.1
Protein GI Number:
4502805
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
.
Swissprot #:
P10645