eIF4E Antibody

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ProSci
Product Code: PSI-79-211
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-211-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209).
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Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209).

Documents

Further Information

Additional Names:
CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, BCL2L3, mRNA cap-binding protein, eIF-4F 25 kDa subunit
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Background References:
  • Li BD, et al. (1998) Ann Surg; 227 (5): 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17 (18): 7520De Gregorio E, et al. (2001) RNA; 7 (1): 106-113Gu W, et al. (2004) Nucleic Acids Res; 32 (15): 4448-4461
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
eIF4E (Phospho-Ser209) antibody was raised against a peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.
Modifications:
Phospho-Specific
NCBI Gene ID #:
1977
NCBI Official Name:
eukaryotic translation initiation factor 4E
NCBI Official Symbol:
EIF4E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
NP_001124150.1
Protein GI Number:
194578907
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.
Swissprot #:
P06730