IkB-epsilon Antibody

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ProSci
Product Code: PSI-79-197
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-197-0.1mg0.1mg£488.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from 293 cells untreated or treated with TNF using IkB-ε (Phospho-Ser22).
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-ε (Phospho-Ser22).
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Western blot analysis of lysed extracts from 293 cells untreated or treated with TNF using IkB-ε (Phospho-Ser22).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-ε (Phospho-Ser22).

Documents

Further Information

Additional Names:
IKBE, BHLHE39, I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Background:
Inhibits NF-κ-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-κ-B p50-p65 and p50-c-Rel complexes.
Background References:
  • Shirane M, et al. (1999) J Biol Chem; 274 (40): 28169-74Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663. Chen, Z. J. et al. (1996) Cell; 84, 853-862. Brown, K. et al. (1995) Science; 267, 1485-1488.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IkB-epsilon (Phospho-Ser22) antibody was raised against a peptide sequence around phosphorylation site of serine 22 (I-E-S (p) -L-R) derived from Human IkB-ε.
Modifications:
Phospho-Specific
NCBI Gene ID #:
4794
NCBI Official Name:
nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon
NCBI Official Symbol:
NFKBIE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
NP_004547.2
Protein GI Number:
71274109
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of IkappaB-ε only when phosphorylated at serine 22.
Swissprot #:
O00221