p70 S6 Kinase Antibody

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ProSci
Product Code: PSI-79-221
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-221-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase (Phospho-Ser411).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser411).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase (Phospho-Ser411).
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Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase (Phospho-Ser411).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser411).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using p70 S6 Kinase (Phospho-Ser411).

Documents

Further Information

Additional Names:
S6K, PS6K, S6K1, STK14A, p70-S6K, p70S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, MACS, PRKCSL, KS6B1, P70-S6K, RPS6KB1, S6K
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.
Background References:
  • Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24): 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484?97
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
p70 S6 Kinase (Phospho-Ser411) antibody was raised against a peptide sequence around phosphorylation site of serine 411 (I-R-S (p) -P-R) derived from Human p70 S6 Kinase.
Modifications:
Phospho-Specific
NCBI Gene ID #:
6198
NCBI Official Name:
ribosomal protein S6 kinase, 70kDa, polypeptide 1
NCBI Official Symbol:
RPS6KB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
70 85 kDa
Protein Accession #:
NP_003152.1
Protein GI Number:
4506737
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at serine 411.
Swissprot #:
P23443