HSP27 Antibody

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ProSci
Product Code: PSI-79-216
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-216-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78).
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Western blot analysis of lysed extracts from HL60 cells untreated or treated with UV using HSP27 (Phospho-Ser78).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27 (Phospho-Ser78).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, nuclear, centrosomal, midbody staining using HSP27 (Phospho-Ser78).

Documents

Further Information

Additional Names:
CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, KIAA0204, STK2, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Involved in stress resistance and actin organization.
Background References:
  • J Landry, et al. (1992) J. Biol. Chem., Vol. 267, Issue 2: 794-803Sita Somara, et al. (2004) Am J Physiol Cell Physiol 286: C1290-C1301Catherine Gaitanaki, et al. (2003) Experimental Biology 206, 2759-2769 (2003) Jing Dong, et al. (2004) Am J Physiol Renal Physiol 287: F1049-F1058
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HSP27 (Phospho-Ser78) antibody was raised against a peptide sequence around phosphorylation site of serine 78 (A-L-S (p) -R-Q) derived from Human HSP27.
Modifications:
Phospho-Specific
NCBI Gene ID #:
3315
NCBI Official Name:
heat shock 27kDa protein 1
NCBI Official Symbol:
HSPB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
NP_001531.1
Protein GI Number:
4504517
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific,Apoptosis,Cancer
SPECIFICITY:
This antibody detects endogenous level of HSP27 only when phosphorylated at serine 78.
Swissprot #:
P04792