IP-10 Antibody

ProSci
Product Code: PSI-38-252
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-252-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea

Images

1 / 4
To detect Rat IP-10 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat IP-10 (38-255) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.
2 / 4
3 / 4
4 / 4
This antibody stained colchicine injected rat brain (including the caudate putamen) tissue.  The primary antibody was incubated at 0.25 ug/ml overnight at 4°C.  This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.  Optimal concentrations and conditions may vary.

To detect Rat IP-10 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat IP-10 (38-255) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.
This antibody stained colchicine injected rat brain (including the caudate putamen) tissue.  The primary antibody was incubated at 0.25 ug/ml overnight at 4°C.  This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent.  Optimal concentrations and conditions may vary.

Further Information

Additional Names:
IP-10, Scyb10, Inp10, Mob1, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Gamma-IP10
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IP-10 (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required.

Sandwich
To detect Rat IP-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IP-10 (38-255) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.

Western Blot:
To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin- β1. Human Heregulin- β1 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
245920
NCBI Official Name:
chemokine (C-X-C motif) ligand 10
NCBI Official Symbol:
Cxcl10
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P48973
Protein GI Number:
146345511
Research Area:
Antibody Pairs, Functional Assays
Swissprot #:
P48973
User NOte:
Centrifuge vial prior to opening.