IGF-BP1 Antibody

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ProSci
Product Code: PSI-38-265
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-265-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea

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To detect hIGF-BP1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IGF-BP1 (38-276) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.
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To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.125 ug/ml- 0.200 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
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To detect hIGF-BP1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IGF-BP1 (38-276) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.125 ug/ml- 0.200 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

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Further Information

Additional Names:
AFBP, IBP1, PP12, IGF-BP25, hIGFBP-1, Insulin-like growth factor-binding protein 1, Placental protein 12, IBP-1
Application Note:
ELISA:
Sandwich:
To detect hIGF-BP1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IGF-BP1 (38-276) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP1.

Western Blot:

To detect hIGF-BP1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human IGF-BP1. Human IGF-BP1 specific antibody was purified by affinity chromatography employing an immobilized Human IGF-BP1 matrix.
NCBI Gene ID #:
3484
NCBI Official Name:
insulin-like growth factor binding protein 1
NCBI Official Symbol:
IGFBP1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P08833
Protein GI Number:
124055
Research Area:
Antibody Pairs
Swissprot #:
P08833
User NOte:
Centrifuge vial prior to opening.