VCAM-1 Antibody

ProSci
Product Code: PSI-38-270
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-270-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea

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To detect hVCAM-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human VCAM-1 (38-281) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1.
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To detect hVCAM-1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human VCAM-1 (38-281) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1.

Further Information

Additional Names:
CD106, INCAM-100, L1CAM, Vascular cell adhesion protein 1, V-CAM 1
Application Note:
ELISA:
Sandwich:
To detect hVCAM-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human VCAM-1 (38-281) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1.

Western Blot:

To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVCAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human VCAM-1. Human VCAM-1 specific antibody was purified by affinity chromatography employing an immobilized Human VCAM-1 matrix.
NCBI Gene ID #:
7412
NCBI Official Name:
vascular cell adhesion molecule 1
NCBI Official Symbol:
VCAM1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P19320
Protein GI Number:
137560
Research Area:
Antibody Pairs
Swissprot #:
P19320
User NOte:
Centrifuge vial prior to opening.