SCF Antibody
| Code | Size | Price |
|---|
| PSI-38-251-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea
Documents
Further Information
Additional Names:
Mgf, SCF, Kitl, Mgf, Kit ligand, Hematopoietic growth factor KL, MGF
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF (50.0 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA:
Indirect:
To detect rat SCF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.
Sandwich
To detect rat SCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat SCF (38-258) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.
Western Blot:
To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF (50.0 ng/mL), a concentration of 1.2-1.8 μg/mL of this antibody is required. ELISA:
Indirect:
To detect rat SCF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.
Sandwich
To detect rat SCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat SCF (38-258) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF.
Western Blot:
To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Rat IP-10. Rat IP-10 specific antibody was purified by affinity chromatography employing an immobilized Rat IP-10 matrix.
NCBI Gene ID #:
60427
NCBI Official Name:
KIT ligand
NCBI Official Symbol:
Kitlg
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P21581
Protein GI Number:
22654283
Research Area:
Antibody Pairs, Functional Assays
Swissprot #:
P21581
User NOte:
Centrifuge vial prior to opening.