IGF-BP7 Antibody

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ProSci
Product Code: PSI-38-249
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-249-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repea

Images

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To detect hIGF-BP7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IGF-BP7 (38-155) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7.
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This antibody stained HeLa and U-251 MG cells. The primary antibody was incubated at 2.0 mg/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
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To detect hIGF-BP7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human IGF-BP7 (38-155) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7.
This antibody stained HeLa and U-251 MG cells. The primary antibody was incubated at 2.0 mg/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Documents

Further Information

Additional Names:
AGM, PSF, TAF, FSTL2, IBP-7, MAC25, IGFBP-7, RAMSVPS, IGFBP-7v, IGFBPRP1, Insulin-like growth factor-binding protein 7, IGFBP-rP1
Application Note:
ELISA:
Indirect:
To detect hIGF-BP7 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7.

Sandwich
To detect hIGF-BP7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IGF-BP7 (38-155) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7.

Western Blot:
To detect hIGF-BP7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Human IGF-BP3 specific antibody was purified by affinity chromatography employing immobilized hIGF-BP3 matrix.
NCBI Gene ID #:
3490
NCBI Official Name:
insulin-like growth factor binding protein 7
NCBI Official Symbol:
IGFBP7
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
Q16270
Protein GI Number:
23396609
Research Area:
Antibody Pairs
Swissprot #:
Q16270
User NOte:
Centrifuge vial prior to opening.