SFPQ Antibody
| Code | Size | Price |
|---|
| PSI-29-346-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SFPQ antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
SFPQ, PSF, POMP100
Application Note:
SFPQ antibody can be used for detection of SFPQ by ELISA at 1:12500. SFPQ antibody can be used for detection of SFPQ by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
SFPQ is DNA- and RNA binding protein. It is essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. It binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. It interacts with U5 snRNA. It may be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. SFPQ may be involved in homologous DNA pairing. SFPQ is involved in transcriptional regulation. It binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity.
Background References:
- Rosonina, E., (2005) Mol. Cell. Biol. 25 (15), 6734-6746.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SFPQ.
NCBI Gene ID #:
6421
NCBI Official Name:
splicing factor proline/glutamine-rich
NCBI Official Symbol:
SFPQ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
78 kDa
Protein Accession #:
NP_005057
Protein GI Number:
4826998
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
RNA Binding ,Cancer
Swissprot #:
P23246
User NOte:
Optimal dilutions for each application to be determined by the researcher.