ARPC3 Antibody

ProSci
Product Code: PSI-45-294
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-294-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-294 staining (1ug/ml) of Mouse Brain lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

45-294 staining (1ug/ml) of Mouse Brain lysate (RIPA buffer, 35ug total protein per lane). Detected by chemiluminescence.

Further Information

Additional Names:
ARPC3, ARC21, p21-Arc, actin related protein 2/3 complex, subunit 3, 21kDa, ARP2/3 protein complex subunit p21, actin related protein 2/3 complex subunit 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Band observed just below 20kDa marker in HeLa, Human Brain and Mouse Brain lysates (predicted MW of 20kDa according to NP_005710). Recommended for use at 1-3ug/ml. Primary incubation was for 1 hour. This antibody has been successfully used in Western blot on Mouse: Fu X et al (2013). PMID: 23303949.
Background References:
  • Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997 Jul 28;138(2):375-84.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RQFMNKSLSGPGQ
NCBI Gene ID #:
10094
NCBI Official Name:
actin related protein 2/3 complex, subunit 3, 21kDa
NCBI Official Symbol:
ARPC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005710.1
Protein GI Number:
5031597
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Please note there are two hypothetical proteins called "similar to the Arp2/3 protein complex subunit p21-Arc" that are virtually identical (CAC14083, XP_208062).
Swissprot #:
O15145