Cytokeratin Monoclonal Antibody

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ProSci
Product Code: PSI-32-143
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-143-0.1mL0.1mL£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 7H8C4
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Cytokeratin(Pan) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using CK monoclonal antibody against truncated CK recombinant protein.
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Western blot analysis using CK monoclonal antibody against truncated CK recombinant protein.

Documents

Further Information

Additional Names:
58 kDa cytokeratin, CK-5, K5, CK5, DDD, DDD1, EBS2, KRT5A
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.
Background References:
  • Scope A. Schwendenwein I. Frommlet F. Vet Rec. 2006, Dec 16, 159(25): 839-43.
  • Somjen D. Katzburg S. Posner GH. et al. J Cell Biochem. 2007, Apr 15, 100(6): 1406-14.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant CK expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
3852
NCBI Official Name:
keratin 5
NCBI Official Symbol:
KRT5
NCBI Organism:
Homo sapiens
Protein Accession #:
P13647
Protein GI Number:
143811411
Swissprot #:
P13647
User NOte:
Optimal dilutions for each application to be determined by the researcher.