Progesterone Receptor Monoclonal Antibody

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ProSci
Product Code: PSI-XPS-1023
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XPS-1023-0.1mL0.1mL£686.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.

Images

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Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020(500nm) showing phosphospecific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190.
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Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020(500nm) showing phosphospecific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190.

Documents

Further Information

Application Note:
As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser190 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB). Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.
Background:
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
Background References:
  • Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L., and Edwards, D.P., "Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody," Mol. Endocrinol., 14 (2000) 52 - 65.
  • Lin, V. C. L., Woon, C. T., Aw, S. E., Guo, C., "Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion," Endocrinology 144 (2003) 5650 -5657.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Progesterone receptor (Ser190) monoclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer190 of human progesterone receptor.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5241
NCBI Official Name:
progesterone receptor
NCBI Official Symbol:
PGR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
90/120
Protein Accession #:
P06401
Protein GI Number:
90110048
Purification:
Protein G purified
Research Area:
Signal Transduction ,Growth Factor ,Phospho-Specific
SPECIFICITY:
Progesterone Receptor antibody is specific for the human progesterone receptor phosphorylated at Ser190.
Swissprot #:
P06401
User NOte:
Optimal dilutions for each application to be determined by the researcher.