ATGL Antibody

ProSci
Product Code: PSI-45-306
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-306-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-306 (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-306 (0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
ATGL, DESNUTRIN, PNPLA2, patatin-like phospholipase domain containing 2, 1110001C14Rik, DKFZp667M109, FP17548, PEDF-R, TTS-2.2, TTS2, adipose triglyceride lipase, pigment epithelium-derived factor, transport-secretion protein 2.2, triglyceride hydrolase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in Human Adipose lysates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Testis for this particular batch.Immunohistochemistry:In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration: 2-3ug/ml.
Background References:
  • Schoenborn V, Heid IM, Vollmert C, Lingenhel A, Adams TD, Hopkins PN, Illig T, Zimmermann R, Zechner R, Hunt SC, Kronenberg F. The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes. Diabetes. 2006 May;55(5):1270-5.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NIIEVSKEARKR
NCBI Gene ID #:
57104
NCBI Official Name:
patatin-like phospholipase domain containing 2
NCBI Official Symbol:
PNPLA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065109.1
Protein GI Number:
21313274
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1.
Swissprot #:
Q96AD5