GRO/MGSA Antibody

ProSci
Product Code: PSI-XP-5147
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5147-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
GRO/MGSA antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated free

Images

1 / 5
To detect Human GRO/MGSA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human GRO/MGSA (XP-5147Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO/MGSA.
2 / 5
To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 5
To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 5
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml?0.150 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
5 / 5
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml?0.150 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

To detect Human GRO/MGSA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Human GRO/MGSA (XP-5147Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO/MGSA.
To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human GRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO/MGSA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml?0.150 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.125 ug/ml?0.150 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.

Further Information

Additional Names:
FSP, GRO1, GROa, MGSA, NAP-3, SCYB1, MGSA-a, GRO, GROA, Growth-regulated alpha protein, C-X-C motif chemokine 1
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hGRO/MGSA 100 ng/mL), a concentration of 1.5 - 3.0 μg/mL of this antibody is required.

ELISA:

To detect hGRO/MGSA by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO/MGSA.

Sandwich: To detect hGRO/MGSA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO/MGSA (XP-5147Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO/MGSA.

Western Blot:
To detect hGRO/MGSA by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO/MGSA is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO/MGSA (human GRO/MGSA).
NCBI Gene ID #:
2919
NCBI Official Name:
chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
NCBI Official Symbol:
CXCL1
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P09341
Protein GI Number:
121622
Purification:
Anti-hGRO/MGSA specific antibody was purified by affinity chromatography employing immobilized hGRO/MGSA matrix.
Research Area:
Signal Transduction,Antibody Pairs, Functional Assays
Swissprot #:
P09341
User NOte:
Centrifuge vial prior to opening.