Bcl-rambo (CT) (Rocky-2) Monoclonal Antibody

ProSci
Product Code: PSI-XA-1031
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XA-1031-0.1mg0.1mg£657.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Bcl-rambo antibody can be stored at 4˚C, stable for one year.

Further Information

Application Note:
Bcl-rambo antibody can be used to recognize the C-terminus of human Bcl-rambo (aa. 224-450). It detects endogenous protein by Immunocytochemistry (1:300), Immunoprecipitation (1:100) and Western Blot (1:1,000). Method: Following fixation with 2% paraformaldehyde/PBS, HELA cells were incubated with MAb to Bcl-rambo (human) (CT) (Rocky-2) at a 1:300 dilution. An anti-mouse IgG antibody conjugated with Alexa Fluor 488 was used for detection. This product is for research use only.
Background References:
  • Bcl-rambo, a novel Bcl-2 homologue that induces apoptosis via its unique C-terminal extension: T. Kataoka, et al.; J. Biol. Chem. 276, 19548 (2001).
  • Bcl-rambo beta, a special splicing variant with an insertion of an Alu-like cassette, promotes etoposide- and Taxol-induced cell death: P. Yi, et al.; FEBS Lett. 534, 61 (2003).
Buffer:
PBS containing 0.02% sodium azide
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Bcl-rambo (monoclonal Rocky-2) antibody was raised against recombinant human Bcl-rambo.
NCBI Gene ID #:
23786
NCBI Official Name:
BCL2-like 13 (apoptosis facilitator)
NCBI Official Symbol:
BCL2L13
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Bcl-rambo (monoclonal Rocky-2) antibody was purified from concentrated hybridoma tissue culture supernatant. Bcl-rambo (monoclonal Rocky-1) antibody was purified to ≥95% in SDS-PAGE.
Research Area:
Apoptosis
Swissprot #:
Q9BXK5
User NOte:
Optimal dilutions for each application to be determined by the researcher.