MEK-2 Antibody

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ProSci
Product Code: PSI-79-106
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-79-106-0.1mg0.1mg£488.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Store antibody at -20˚C for up to one year.

Images

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Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).
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<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2 (Phospho-Thr394).
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Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2 (Phospho-Thr394).

Documents

Further Information

Additional Names:
CFC4, MEK2, MKK2, MAPKK2, PRKMK2, SRC1, ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background:
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Background References:
  • Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221. Cowley S, et al. (1994) Cell. 77:841-852.
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MEK-2 (Phospho-Thr394) antibody was raised against a peptide sequence around phosphorylation site of threonine 394 (P-G-T (p) -P-T) derived from Human MEK-2.
Modifications:
Phospho-Specific
NCBI Gene ID #:
5605
NCBI Official Name:
mitogen-activated protein kinase kinase 2
NCBI Official Symbol:
MAP2K2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
NP_109587.1
Protein GI Number:
13489054
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Research Area:
Phospho-Specific
SPECIFICITY:
This antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.
Swissprot #:
P36507