Amisyn Antibody

ProSci
Product Code: PSI-45-250
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-250-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
45-250 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

45-250 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
STXBP6, syntaxin binding protein 6 (amisyn), amisyn, HSPC156, amisyn, syntaxin binding protein 6, FLJ39638, OTTHUMP00000178754
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave bands at both approx 25kDa and approx 45kDa in Human Brain extracts at 0.1ug/ml. Please note that currently we cannot find an explanation in the literature for the 45kDa band we observe given the predicted size of approx. 24Kda according to NP_054897. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasm staining of PCT and DCT cells Recommended concentration, 3-5ug/ml.
Background References:
  • Scales SJ, Hesser BA, Masuda ES, Scheller RH. Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly. J Biol Chem. 2002 Aug 2;277(31):28271-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: SAKSAISKEIFAP-C
NCBI Gene ID #:
29091
NCBI Official Name:
syntaxin binding protein 6 (amisyn)
NCBI Official Symbol:
STXBP6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_054897.4
Protein GI Number:
21426793
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q8NFX7