AKT1 Antibody
| Code | Size | Price |
|---|
| PSI-31-052-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store AKT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
AKT1, AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
Application Note:
AKT1 antibody can be used for detection of AKT1 by ELISA at 1:312500. AKT1 antibody can be used for detection of AKT1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Background References:
- Kim, H.A., et al., (2006) Exp. Mol. Pathol. 80 (2), 165-170.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKT1.
NCBI Gene ID #:
207
NCBI Official Name:
v-akt murine thymoma viral oncogene homolog 1
NCBI Official Symbol:
AKT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
NP_005154
Protein GI Number:
62241011
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Cancer ,Signal Transduction ,Apoptosis
Swissprot #:
P31749
User NOte:
Optimal dilutions for each application to be determined by the researcher.