EDF1 Antibody

ProSci
Product Code: PSI-28-730
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-730-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EDF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Jurkat 1.25 ug/ml.

Antibody used in WB on Human Jurkat 1.25 ug/ml.

Further Information

Additional Names:
EDF1, MBF1, EDF-1
Application Note:
EDF1 antibody can be used for detection of EDF1 by ELISA at 1:312500. EDF1 antibody can be used for detection of EDF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene.
Background References:
  • Brendel, C., et al., (2002) Mol. Endocrinol. 16 (6), 1367-1377.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EDF1.
NCBI Gene ID #:
8721
NCBI Official Name:
endothelial differentiation-related factor 1
NCBI Official Symbol:
EDF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
16 kDa, 15 kDa
Protein Accession #:
NP_003783
Protein GI Number:
4503453
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription
Swissprot #:
O60869
User NOte:
Optimal dilutions for each application to be determined by the researcher.