SMARCA3 Antibody

ProSci
Product Code: PSI-28-113
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-113-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMARCA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Transfected 293T at 2.5 ug/ml.

Antibody used in WB on Transfected 293T at 2.5 ug/ml.

Further Information

Additional Names:
SMARCA3, ZBU1, HLTF1, RNF80, HIP116, SNF2L3, HIP116A, SMARCA3
Application Note:
SMARCA3 antibody can be used for detection of SMARCA3 by ELISA at 1:312500. SMARCA3 antibody can be used for detection of SMARCA3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
SMARCA3 (HLTF) is a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA3 contains a RING finger DNA binding motif. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform which is truncated at the N-terminus as compared to the full-length protein.
Background References:
  • Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMARCA3.
NCBI Gene ID #:
6596
NCBI Official Name:
helicase-like transcription factor
NCBI Official Symbol:
HLTF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
114 kDa, 114 kDa
Protein Accession #:
NP_620636
Protein GI Number:
21071054
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription
Swissprot #:
Q14527
User NOte:
Optimal dilutions for each application to be determined by the researcher.