TOP2A Antibody
| Code | Size | Price |
|---|
| PSI-31-047-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TOP2A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
TOP2A, TOP2, TP2A
Application Note:
TOP2A antibody can be used for detection of TOP2A by ELISA at 1:62500. TOP2A antibody can be used for detection of TOP2A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Background References:
- Yoshida, K., et al., (2006) Mol. Cell. Biol. 26 (9), 3414-3431.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TOP2A.
NCBI Gene ID #:
7153
NCBI Official Name:
topoisomerase (DNA) II alpha 170kDa
NCBI Official Symbol:
TOP2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
174 kDa
Protein Accession #:
NP_001058
Protein GI Number:
19913406
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cancer
Swissprot #:
P11388
User NOte:
Optimal dilutions for each application to be determined by the researcher.