PTRF Antibody

ProSci
Product Code: PSI-28-592
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-592-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PTRF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Mouse embryonic fibroblast at: 1:1000 (Lane 1: 50ug mouse embryonic fibroblast lysate, Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate, Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein).
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Antibody used in IHC on Mouse at of 5 ug/ml.

Antibody used in WB on Mouse embryonic fibroblast at: 1:1000 (Lane 1: 50ug mouse embryonic fibroblast lysate, Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate, Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein).
Antibody used in IHC on Mouse at of 5 ug/ml.

Further Information

Additional Names:
PTRF, Cavin, Cav-p60, AW546441, 2310075E07Rik
Application Note:
PTRF antibody can be used for detection of PTRF by ELISA at 1:312500. PTRF antibody can be used for detection of PTRF by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
In mouse, pausing of elongation complexes is mediated by the transcription termination factor TTF-I bound to the 'Sal box' terminator downstream of the rDNA transcription unit. PTRF is a Pol I and transcript release factor for dissociation of paused ternary complexes.
Background References:
  • Miyoshi, K., et al., (2001) Genomics 71 (2), 150-155.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse PTRF.
NCBI Gene ID #:
19285
NCBI Official Name:
polymerase I and transcript release factor
NCBI Official Symbol:
PTRF
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
NP_033012
Protein GI Number:
6679567
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription
Swissprot #:
O54724
User NOte:
Optimal dilutions for each application to be determined by the researcher.