SFPI1 Antibody

ProSci
Product Code: PSI-28-754
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-28-754-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SFPI1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Lymphnode at 5.0 ug/ml.

Antibody used in WB on Human Lymphnode at 5.0 ug/ml.

Further Information

Additional Names:
SFPI1, Dis1, PU.1, Dis-1, Sfpi1, Spi-1, Sfpi-1, Tcfpu1, Tfpu.1
Application Note:
SFPI1 antibody can be used for detection of SFPI1 by ELISA at 1:312500. SFPI1 antibody can be used for detection of SFPI1 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
Sfpi1 binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Sfpi1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Sfpi1 also binds RNA and may modulate pre-mRNA splicing.
Background References:
  • McDevit, D.C. (2006) Mol. Immunol. 43 (10), 1541-1548.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse SFPI1.
NCBI Gene ID #:
20375
NCBI Official Name:
spleen focus forming virus (SFFV) proviral integration oncogene
NCBI Official Symbol:
Spi1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
NP_035485
Protein GI Number:
6755474
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription
Swissprot #:
P17433
User NOte:
Optimal dilutions for each application to be determined by the researcher.