BOK Antibody

ProSci
Product Code: PSI-42-325
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-325-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-325 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

42-325 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
BOK, BCL2-related ovarian killer, BCL2L9, BOKL, MGC4631
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave bands at approx 55kDa and 20kDa in Human Testis lysates after 1ug/ml antibody staining, and 17kDa in Mouse Spleen and Thymus lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 23.3kDa according to NP_115904.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Cow
Immunogen:
The immunogen for this antibody is: C-HISLQSEPVVTD
NCBI Gene ID #:
666
NCBI Official Name:
BCL2-related ovarian killer
NCBI Official Symbol:
BOK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_115904.1
Protein GI Number:
14210524
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Apoptosis
Swissprot #:
Q9UMX3