C3 Antibody
| Code | Size | Price |
|---|
| PSI-43-099-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
C3, complement component 3, AHUS5, ARMD9, ASP, CPAMD1, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, OTTHUMP00000197086, acylation-stimulating protein cleavage product, complement C3, complement component C3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 125kDa band in Human Liver lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104kDa according to the largest fragment C3 alpha chain (aa672-1663). Although this fragment is glycosylated, we have not yet found support in the literature for the observed size. The 125kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field. Please note that a different antibody developed to a different part of the protein showed identical results: EB11703.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: DKVGKYPKELRKC
NCBI Gene ID #:
718
NCBI Official Name:
complement component 3
NCBI Official Symbol:
C3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 125 kDa
Protein Accession #:
NP_000055.2
Protein GI Number:
115298678
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize the C3 alpha chain and C3a anaphylatoxin,
Swissprot #:
P01024