DAGLA Antibody

ProSci
Product Code: PSI-46-829
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-829-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-829 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-829 (5ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
DAGLA, diacylglycerol lipase, alpha, C11orf11, DAGL(ALPHA), DAGLALPHA, NSDDR, diacylglycerol lipase alpha, neural stem cell-derived dendrite regulator, KIAA0659
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 125kDa band observed in Human Liver lysates (calculated MW of 115kDa according to NP_006124.1). Recommended concentration: 0.3-1ug/ml. Some minor background is detected and is blocked by the immunizing peptide. Preliminary testing was unsuccessful on Mouse for this particular batch.Immunohistochemistry:Paraffin embedded Human Breast, Pancreas and Spleen. Recommended concentration: 5ug/ml.
Background References:
  • Bisogno T, Howell F, Williams G, Minassi A, Cascio MG, Ligresti A, Matias I, Schiano-Moriello A, Paul P, Williams EJ, Gangadharan U, Hobbs C, Di Marzo V, Doherty P. Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain. J Cell Biol. 2003 Nov 10;163(3):463-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-PAKQDELVISAR
NCBI Gene ID #:
747
NCBI Official Name:
diacylglycerol lipase, alpha
NCBI Official Symbol:
DAGLA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006124.1
Protein GI Number:
27262632
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Neuroscience ,Obesity
SPECIFICITY:
.
Swissprot #:
Q9Y4D2