GEM Antibody

ProSci
Product Code: PSI-43-052
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-43-052-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with 43-052 (1ug/ml). The same lysates probed with anti-HA tag antibody in the lo
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HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with 43-052 (1ug/ml). The same lysates probed with anti-HA tag antibody in the lo

Further Information

Additional Names:
GEM, GTP binding protein overexpressed in skeletal muscle, KIR, MGC26294, GTP-binding mitogen-induced T-cell protein, GTP-binding protein GEM, GTP-binding protein overexpressed in skeletal muscle, OTTHUMP00000226600, OTTHUMP00000226601, RAS-like protein KIR, kinase-inducible Ras-like protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing Human recombinant GEM bands of approx. 35+45kDa are observed. These bands are not observed in the transfected HEK293 with empty vector and the antibody does not cross-react to related protein such as Rem, Rem2L or Rad. Data provided by Prof. R Andres, Univ. Kentucky, USA. A similar banding pattern we observed in Human Spleen. The calculated molecular size is 33.9kDa according to NP_005252.1. Recommended concentration: 1-3ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QKEPHQYSHRNRH
NCBI Gene ID #:
2669
NCBI Official Name:
GTP binding protein overexpressed in skeletal muscle
NCBI Official Symbol:
GEM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005252.1
Protein GI Number:
4885263
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
Reported variants represent identical protein: NP_005252.1, NP_859053.1
Swissprot #:
P55040