GRIK3 Antibody

ProSci
Product Code: PSI-46-854
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-854-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
46-854(0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

46-854(0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
GRIK3, GLUR7, glutamate receptor, ionotropic, kainate 3, EAA5, GLR7, GluR7a, dJ1090M5.1 (glutamate receptor, ionotropic, kainate 3 (GLUR7)), excitatory amino acid receptor 5, glutamate receptor 7
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 104kDa according to Human NP_000822.2, and to Rat NP_001106187.1). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 39kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Puranam RS, Eubanks JH, Heinemann SF, McNamara JO. Chromosomal localization of gene for human glutamate receptor subunit-7. Somat Cell Mol Genet. 1993 Nov;19(6):581-8
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-KIRQLPIDSDDSRP
NCBI Gene ID #:
2899
NCBI Official Name:
glutamate receptor, ionotropic, kainate 3
NCBI Official Symbol:
GRIK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000822.2
Protein GI Number:
28605145
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Neuroscience
SPECIFICITY:
.
Swissprot #:
Q13003