Keratin 13 Antibody

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ProSci
Product Code: PSI-46-751
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-751-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-751 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-751 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
KRT13, keratin 13, CK13, K13, MGC161462, MGC3781, cytokeratin 13, keratin, type I cytoskeletal 13
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 55kDa band observed in Human Lung, Skeletal Muscle and Skin lysates (calculated MW of 49.6kDa according to NP_705694.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sajjan, Microbiology. 2003 Apr;149(Pt 4):961-71.; PMID: 12686638). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Tonsil shows strong layered, filamentous staining. Recommended concentration, 2-4ug/ml.
Background References:
  • Matta H, Mazzacurati L, Schamus S, Yang T, Sun Q, Chaudhary PM. Kaposi's sarcoma-associated herpesvirus (KSHV) oncoprotein K13 bypasses TRAFs and directly interacts with the IkappaB kinase complex to selectively activate NF-kappaB without JNK activation. J Biol Chem. 2007 Aug 24;282(34):24858-65.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TSNASGRRTSDVRRP
NCBI Gene ID #:
3860
NCBI Official Name:
keratin 13
NCBI Official Symbol:
KRT13
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_705694.2
Protein GI Number:
131412225
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognize the reported isoform a (NP_002265.2) only.
Swissprot #:
P13646