Mammaglobin A Antibody

ProSci
Product Code: PSI-45-859
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-859-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-859 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

45-859 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
SCGB2A2, secretoglobin, family 2A, member 2 , HGNC:7050, MGB1, UGB2, MGC71974, mammaglobin 1, mammaglobin A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the calculated size of 10.5kDa according to NP_002402.1. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Breast shows staining of cytoplasm in some acinar cells.. Recommended concentration, 4-6ug/ml.
Background References:
  • Ouellette RJ, Richard D, Maicas E. RT-PCR for mammaglobin genes, MGB1 and MGB2, identifies breast cancer micrometastases in sentinel lymph nodes. Am J Clin Pathol. 2004 May;121(5):637-43. Erratum in: Am J Clin Pathol. 2004 Nov;122(5):813.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-NPQVSKTEYKEL
NCBI Gene ID #:
4250
NCBI Official Name:
secretoglobin, family 2A, member 2
NCBI Official Symbol:
SCGB2A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002402.1
Protein GI Number:
4505169
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q13296