MSH2 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-196-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3A2B8C
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
MSH2 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
MutS protein homolog 2, hMSH2, FCC1, COCA1, HNPCC, LCFS2, HNPCC1
Application Note:
Western Blot:Dilution 1:200 - 1:1,000
IHC(P):Dilution 1:200 - 1:1,000
IHC(F):Dilution 1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
IHC(P):Dilution 1:200 - 1:1,000
IHC(F):Dilution 1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.
Background References:
- Papadopoulos, N. 1994. Science 263: 1625-1629.
- Palombo, F. 1994. Nature 367:417-418.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
4436
NCBI Official Name:
mutS homolog 2
NCBI Official Symbol:
MSH2
NCBI Organism:
Homo sapiens
Protein Accession #:
P43246
Protein GI Number:
1171032
Swissprot #:
P43246
User NOte:
Optimal dilutions for each application to be determined by the researcher.