NPM1 Monoclonal Antibody
| Code | Size | Price |
|---|
| PSI-32-198-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 7H10B9
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
NPM1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
NPM, Nucleolar phosphoprotein B23, NPM, B23
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Nucleophosmin (NPM), also named B23 or NO38, is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. NPM1 has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities . The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase .
Background References:
- Morris SW, et al. Science 1994 Mar 4; 263(5151):1281-4.
- Yoneda-Kato N, et al. Oncogene. 1996 Jan 18;12(2):265-75.
- Liu HT, Yung BY. Cancer Lett. 1999 Sep 20;144(1):45-54.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant human NPM1 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
4869
NCBI Official Name:
nucleophosmin (nucleolar phosphoprotein B23, numatrin)
NCBI Official Symbol:
NPM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
P06748
Protein GI Number:
114762
Purification:
Antibody is purified by protein A affinity chromatography.
Swissprot #:
P06748
User NOte:
Optimal dilutions for each application to be determined by the researcher.