MCL1 Monoclonal Antibody
Code | Size | Price |
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PSI-32-191-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 8C6D4B1
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
MCL-1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MCL1-ES, bcl2-L-3, mcl1/EAT
Application Note:
Western Blot:Dilution 1:200 - 1:1,000
IHC(P):Dilution 1:200 - 1:1,000
IHC(F):Dilution 1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
IHC(P):Dilution 1:200 - 1:1,000
IHC(F):Dilution 1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.
Background References:
- Ota, N. et al. J. Hum. Genet. 2000. 46: 254-269.
- Schwertfeger KL, Ryder JW, Anderson SM J Mammary Gland Biol Neoplasia 2000, 3 : 236-251.
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified recombinant protein MCL-1expressed in E. Coli strain M15.
NCBI Gene ID #:
4170
NCBI Official Name:
myeloid cell leukemia sequence 1 (BCL2-related)
NCBI Official Symbol:
MCL1
NCBI Organism:
Homo sapiens
Protein Accession #:
NP_001184249.1
Protein GI Number:
309747067
Purification:
Antibody is purified by protein G affinity chromatography.
Research Area:
Apoptosis
Swissprot #:
Q07820
User NOte:
Optimal dilutions for each application to be determined by the researcher.